Isolation and screening of diesel-degrading bacteria from the diesel contaminated seawater at Kenjeran Beach, Surabaya

Pratiwi Putri Pranowo*, Harmin Sulistiyaning Titah

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Samples of contaminated seawater by diesel were taken at Kenjeran Beach Surabaya using aseptic technique. Isolation was conducted using serial dilution and spread method on nutrient agar (NA) media. The all bacteria colony were devided in to group based on with morphological characterization and gram staining. After that, those bacterial colonies were tested individually in NA media containing different concentration of diesel (2, 4, 6, 8, and 10%) for up to 7 days at 30°C. The results showed that eight bacterial strains were isolated from diesel contaminated seawater in Kenjeran Beach Surabaya. Screening on diesel showed that all the isolation bacteria were capable of degrading diesel and bacteria with code of B and E haves highly percentage growth in compared to other bacterial isolation. In conclusion, bacteria with code of B and E have potential to be used in diesel bioremediation in contaminated seawater.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalEnvironmentAsia
Volume9
Issue number2
Publication statusPublished - Jul 2016

Keywords

  • Bacteria
  • Colony
  • Diesel
  • Isolate
  • Seawater

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