TY - JOUR
T1 - Isolation and screening of resistant bacteria of heavy metal (Fe) at ship dismantling
AU - Titah, Harmin Sulistiyaning
AU - Pratikno, Herman
AU - Moesriati, Atiek
AU - Putera, Rizky Islami
AU - Imron, Muhammad Fauzul
N1 - Publisher Copyright:
© The Authors, published by EDP Sciences, 2018.
PY - 2018/9/12
Y1 - 2018/9/12
N2 - The activity of ship dismantling is one of the sources of metal pollutant that polluted the environment. The aims of this study were analyze the iron concentration from the ship dismantling area, to isolate the bacteria from those area, and to determine the bacteria resistant on iron. Samplings was conducted in three point sampling at sea water and soil coast, respectively. Isolation of bacteria was carried out using pour plate methods. All isolated bacteria in seawater and soil samples were inoculated on nutrient agar medium (NA) containing ferrous metals (Fe2+) with various concentrations (0; 1,000; 2,000; 3,000; 4,000; 5,000; and 6,000 μg/mL). Based on the results, sea water and soil indicated that those area have contaminated with iron. The concentration of iron in seawater was 1.03, 1.01 and 1.00 μg/mL, respectively. Meanwhile, the concentration of iron in soil was 962.0, 966.05, 981.00 mg/kg, respectively. The result of qualitative and quantitative analysis showed that the isolates of AT, AL and CL coded bacteria have high resistance to the effect of iron. It indicated with clear zone of 6.00-7.00 mm at 6,000 μg/mL. In conclusion, both of bacteria are potential to be used for bioremediation of the ferrous metal (Fe2+) in further investigation.
AB - The activity of ship dismantling is one of the sources of metal pollutant that polluted the environment. The aims of this study were analyze the iron concentration from the ship dismantling area, to isolate the bacteria from those area, and to determine the bacteria resistant on iron. Samplings was conducted in three point sampling at sea water and soil coast, respectively. Isolation of bacteria was carried out using pour plate methods. All isolated bacteria in seawater and soil samples were inoculated on nutrient agar medium (NA) containing ferrous metals (Fe2+) with various concentrations (0; 1,000; 2,000; 3,000; 4,000; 5,000; and 6,000 μg/mL). Based on the results, sea water and soil indicated that those area have contaminated with iron. The concentration of iron in seawater was 1.03, 1.01 and 1.00 μg/mL, respectively. Meanwhile, the concentration of iron in soil was 962.0, 966.05, 981.00 mg/kg, respectively. The result of qualitative and quantitative analysis showed that the isolates of AT, AL and CL coded bacteria have high resistance to the effect of iron. It indicated with clear zone of 6.00-7.00 mm at 6,000 μg/mL. In conclusion, both of bacteria are potential to be used for bioremediation of the ferrous metal (Fe2+) in further investigation.
UR - http://www.scopus.com/inward/record.url?scp=85053810155&partnerID=8YFLogxK
U2 - 10.1051/matecconf/201819713020
DO - 10.1051/matecconf/201819713020
M3 - Conference article
AN - SCOPUS:85053810155
SN - 2261-236X
VL - 197
JO - MATEC Web of Conferences
JF - MATEC Web of Conferences
M1 - 13020
T2 - 3rd Annual Applied Science and Engineering Conference, AASEC 2018
Y2 - 18 April 2018
ER -