Isolation of oryzanol from crude rice bran oil

Siti Zullaikah, Elda Melwita, Yi Hsu Ju*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)


The isolation of oryzanol from crude rice bran oil (RBO) was achieved by a two-step crystallization process. In the first crystallization, oryzanol was concentrated in the liquid phase along with free fatty acid (FFA), monoacylglycerol (MG), squalene, tocols, and phytosterols, whereas the solid phase contained mainly triacylglycerol (TG) and steryl esters. Oryzanol-rich product obtained from the first crystallization was subjected to the second crystallization where the oryzanol-rich product was kept at room temperature (20.5 ± 1.5 °C) for 24 h. Hexane was added as anti-solvent to the oryzanol-rich product and kept at 5 ± 1 °C for another 48 h. Parameters that affect the isolation of oryzanol from crude RBO were systematically investigated. Under optimal operation conditions, oryzanol with purity and recovery of 93-95% and 59%, respectively, was obtained from RBO with an initial FFA content of ca. 5%.

Original languageEnglish
Pages (from-to)299-302
Number of pages4
JournalBioresource Technology
Issue number1
Publication statusPublished - Jan 2009
Externally publishedYes


  • Bioactive compounds
  • Crystallization
  • Oryzanol
  • Rice bran oil


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