9 Citations (Scopus)

Abstract

Lipase is an enzyme that is often used in industry and become a commercial enzyme. One group of microorganisms capable of producing lipase is a yeast. This study aims to screen yeast from Wonorejo mangrove that potential to produce lipase and to optimize the production of these enzymes. Screening test include the measurement of lipolytic index and value of fatty acid. Yeast with the best value of fatty acid will be continued to the measurement of lipase activity. It is affected by several environmental factors, such as pH, temperature, and incubation time. This research was conducted to observe the optimization variation on environmental factors combination to produce lipase. Lipase activity was tested by using p-Nitrophenyl Palmitate (pNPP). Absorbency was measured by spectrofotometer on wavelength of 410 nm. Measurement of the enzyme activity was done by interpolating the absorbance values on the p-nitrophenol standard curve then calculated by the formula. All data were analyzed by using descriptive quantitative method. The results show that the highest lypolityc index was 2.08. The highest value of fatty acid was 0.49 that was reached on 168 hours of incubation. Candida W3.8 expressed the highest lypolylitic potential. The optimum environment to produce lipase by Candida W 3.8 was on 120 hours of incubation time, in temperature range of 27°C - 45°C and pH range of 4,5 - 7.

Original languageEnglish
Title of host publicationProceeding of International Biology Conference 2016
Subtitle of host publicationBiodiversity and Biotechnology for Human Welfare
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415287
DOIs
Publication statusPublished - 26 Jun 2017
Event3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016 - Surabaya, Indonesia
Duration: 15 Oct 2016 → …

Publication series

NameAIP Conference Proceedings
Volume1854
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016
Country/TerritoryIndonesia
CitySurabaya
Period15/10/16 → …

Keywords

  • Wonorejo mangrove
  • enzyme activity
  • lipase
  • yeast

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