Lock-in measurement technique in fluorescent instrumentation system for cell-based odor sensor

Totok Mujiono, Yuji Sukekawa, Takamichi Nakamoto*, Hidefumi Mitsuno, Yuko Nakajima, Ryohei Kanzaki, Nobuo Misawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A fluorescent instrumentation system is widely used for cell-based odor sensors. However, the fluorescent instrumentation system was easily affected by ambient light. The measurement is limited if dark conditions are required for the measurement. This paper presents the design and implementation of a lock-in measurement technique in a fluorescent instrumentation system for a cell-based odor sensor. A continuous mode of laser diode excitation was changed to a pulse mode for the purpose of modulating the fluorescent light. The ambient light asynchronous with the modulated fluorescent light can be eliminated even if the ambient light level is much higher than that of the fluorescent light. Experimental results show that using the lock-in measurement technique, the weak fluorescent signal can be extracted from a modulated signal buried under noise. We conclude that the developed fluorescent measurement system can be used to observe the concentration dependency of odorant even under ambient light conditions.

Original languageEnglish
Pages (from-to)83-89
Number of pages7
JournalIEEJ Transactions on Sensors and Micromachines
Issue number3
Publication statusPublished - 2016
Externally publishedYes


  • Biosensor
  • Fluorescent instrumentation
  • Lock-in measurement technique
  • Odor sensor
  • Olfactory receptors


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