Monomorphic allele rs109231213 in 3’UTR PLAG1 gene in purebred of Bali cattle (Bos javanicus)

Sutikno Sutikno; Jean Pierre Munyaneza; Santiananda Arta Asmarasari; Jakaria Jakaria

doi:10.1051/e3sconf/202233500010

Monomorphic allele rs109231213 in 3’UTR PLAG1 gene in purebred of Bali cattle (Bos javanicus)

Sutikno Sutikno^*, Jean Pierre Munyaneza, Santiananda Arta Asmarasari, Jakaria Jakaria

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

Abstract

The mutation rs109231213 that is located in 3’UTR of PLAG1 gene is associated with the growth and body weight in several Bos taurus and Bos indicus breeds. This study aimed to identify SNP rs109231213 in Bali cattle (Bos javanicus). The study used 41 samples of Bali cattle. The PLAG1 gene polymorphism was analyzed using PCR and direct sequencing methods. PCR pimers were 5’- TTGCACAGAATCAGTGTGTC-3’ and 5’-AGCCTAACGTGGATCTATGG-3’. The results showed that primers successfully amplified the 331 bp fragment at annealing 60⁰C that contained rs109231213. SNP was monomorphic in Bali cattle with one allele (G). This study concludes that rs109231213 in 3’UTR of PLAG1 gene can be used as specific marker in purebred of Bali cattle that have never been crossed with Bos taurus and Bos indicus.

Original language	English
Article number	00010
Journal	E3S Web of Conferences
Volume	335
DOIs	https://doi.org/10.1051/e3sconf/202233500010
Publication status	Published - 14 Jan 2022
Externally published	Yes
Event	2nd International Conference on Environmentally Sustainable Animal Industry, ICESAI 2021 - Malang, Indonesia Duration: 21 Oct 2021 → …

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@article{efb9eaab7ec2429ea29fd54daac3605b,

title = "Monomorphic allele rs109231213 in 3{\textquoteright}UTR PLAG1 gene in purebred of Bali cattle (Bos javanicus)",

abstract = "The mutation rs109231213 that is located in 3{\textquoteright}UTR of PLAG1 gene is associated with the growth and body weight in several Bos taurus and Bos indicus breeds. This study aimed to identify SNP rs109231213 in Bali cattle (Bos javanicus). The study used 41 samples of Bali cattle. The PLAG1 gene polymorphism was analyzed using PCR and direct sequencing methods. PCR pimers were 5{\textquoteright}- TTGCACAGAATCAGTGTGTC-3{\textquoteright} and 5{\textquoteright}-AGCCTAACGTGGATCTATGG-3{\textquoteright}. The results showed that primers successfully amplified the 331 bp fragment at annealing 600C that contained rs109231213. SNP was monomorphic in Bali cattle with one allele (G). This study concludes that rs109231213 in 3{\textquoteright}UTR of PLAG1 gene can be used as specific marker in purebred of Bali cattle that have never been crossed with Bos taurus and Bos indicus.",

author = "Sutikno Sutikno and Munyaneza, {Jean Pierre} and Asmarasari, {Santiananda Arta} and Jakaria Jakaria",

note = "Publisher Copyright: {\textcopyright} The Authors, published by EDP Sciences.; 2nd International Conference on Environmentally Sustainable Animal Industry, ICESAI 2021 ; Conference date: 21-10-2021",

year = "2022",

month = jan,

day = "14",

doi = "10.1051/e3sconf/202233500010",

language = "English",

volume = "335",

journal = "E3S Web of Conferences",

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T1 - Monomorphic allele rs109231213 in 3’UTR PLAG1 gene in purebred of Bali cattle (Bos javanicus)

AU - Sutikno, Sutikno

AU - Munyaneza, Jean Pierre

AU - Asmarasari, Santiananda Arta

AU - Jakaria, Jakaria

N1 - Publisher Copyright: © The Authors, published by EDP Sciences.

PY - 2022/1/14

Y1 - 2022/1/14

N2 - The mutation rs109231213 that is located in 3’UTR of PLAG1 gene is associated with the growth and body weight in several Bos taurus and Bos indicus breeds. This study aimed to identify SNP rs109231213 in Bali cattle (Bos javanicus). The study used 41 samples of Bali cattle. The PLAG1 gene polymorphism was analyzed using PCR and direct sequencing methods. PCR pimers were 5’- TTGCACAGAATCAGTGTGTC-3’ and 5’-AGCCTAACGTGGATCTATGG-3’. The results showed that primers successfully amplified the 331 bp fragment at annealing 600C that contained rs109231213. SNP was monomorphic in Bali cattle with one allele (G). This study concludes that rs109231213 in 3’UTR of PLAG1 gene can be used as specific marker in purebred of Bali cattle that have never been crossed with Bos taurus and Bos indicus.

AB - The mutation rs109231213 that is located in 3’UTR of PLAG1 gene is associated with the growth and body weight in several Bos taurus and Bos indicus breeds. This study aimed to identify SNP rs109231213 in Bali cattle (Bos javanicus). The study used 41 samples of Bali cattle. The PLAG1 gene polymorphism was analyzed using PCR and direct sequencing methods. PCR pimers were 5’- TTGCACAGAATCAGTGTGTC-3’ and 5’-AGCCTAACGTGGATCTATGG-3’. The results showed that primers successfully amplified the 331 bp fragment at annealing 600C that contained rs109231213. SNP was monomorphic in Bali cattle with one allele (G). This study concludes that rs109231213 in 3’UTR of PLAG1 gene can be used as specific marker in purebred of Bali cattle that have never been crossed with Bos taurus and Bos indicus.

UR - http://www.scopus.com/inward/record.url?scp=85146725752&partnerID=8YFLogxK

U2 - 10.1051/e3sconf/202233500010

DO - 10.1051/e3sconf/202233500010

M3 - Conference article

AN - SCOPUS:85146725752

SN - 2267-1242

VL - 335

JO - E3S Web of Conferences

JF - E3S Web of Conferences

M1 - 00010

T2 - 2nd International Conference on Environmentally Sustainable Animal Industry, ICESAI 2021

Y2 - 21 October 2021

ER -

Monomorphic allele rs109231213 in 3’UTR PLAG1 gene in purebred of Bali cattle (Bos javanicus)

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