TY - JOUR
T1 - New potential and characterization of Andrographis paniculata L. Ness plant extracts as photoprotective agent
AU - Fardiyah, Qonitah
AU - Ersam, Taslim
AU - Suyanta,
AU - Slamet, Agus
AU - Suprapto,
AU - Kurniawan, Fredy
N1 - Publisher Copyright:
© 2020 The Author(s)
PY - 2020/12
Y1 - 2020/12
N2 - The species Andrographis paniculata L. Ness, family Acanthaceae grows in many parts in the island of Java in Indonesia. In this research, new potentials of photoprotective agents from A. paniculata plant extracts, along with their characteristics, were investigated. The phytochemical test, infrared analysis, ultraviolet characterization, fluorescence identification, flavonoid content by thin layer chromatography, high performance liquid chromatography-mass spectrometry (LCMS/MS) and photoprotective analysis were performed in the extracts. The new potentials of A. paniculata plant extracts were studied at various concentrations of extracts and shown by the value of the sun protection factor (SPF). Based on phytochemical test, A. paniculata plant extracts contained flavonoids, alkaloids, tannins, triterpenoids and polyphenols. A. paniculata plant extracts have 2 maximum wavelengths at 230 nm and 362 nm. The excitation peak at 300 nm (552.11 a.u) and the emission peak at 605 nm (516.02 a.u). The flavonoid content of the extracts was 0.022 µg/mL quercetin with Rf value of 0.61. The chromatogram of the LCMS/MS is similar to quercetin-3-glycoside standard at a retention time of 2.77. The photoprotective activity of A. paniculata plant extracts had ≥ 15 SPF. Its shows the possibility to use this extract as photoprotective agent in pharmaceutical preparations.
AB - The species Andrographis paniculata L. Ness, family Acanthaceae grows in many parts in the island of Java in Indonesia. In this research, new potentials of photoprotective agents from A. paniculata plant extracts, along with their characteristics, were investigated. The phytochemical test, infrared analysis, ultraviolet characterization, fluorescence identification, flavonoid content by thin layer chromatography, high performance liquid chromatography-mass spectrometry (LCMS/MS) and photoprotective analysis were performed in the extracts. The new potentials of A. paniculata plant extracts were studied at various concentrations of extracts and shown by the value of the sun protection factor (SPF). Based on phytochemical test, A. paniculata plant extracts contained flavonoids, alkaloids, tannins, triterpenoids and polyphenols. A. paniculata plant extracts have 2 maximum wavelengths at 230 nm and 362 nm. The excitation peak at 300 nm (552.11 a.u) and the emission peak at 605 nm (516.02 a.u). The flavonoid content of the extracts was 0.022 µg/mL quercetin with Rf value of 0.61. The chromatogram of the LCMS/MS is similar to quercetin-3-glycoside standard at a retention time of 2.77. The photoprotective activity of A. paniculata plant extracts had ≥ 15 SPF. Its shows the possibility to use this extract as photoprotective agent in pharmaceutical preparations.
KW - Ethanolic extracts
KW - Herbal plant
KW - SPF
KW - Sambiloto
KW - UV protector
UR - http://www.scopus.com/inward/record.url?scp=85095564997&partnerID=8YFLogxK
U2 - 10.1016/j.arabjc.2020.10.015
DO - 10.1016/j.arabjc.2020.10.015
M3 - Article
AN - SCOPUS:85095564997
SN - 1878-5352
VL - 13
SP - 8888
EP - 8897
JO - Arabian Journal of Chemistry
JF - Arabian Journal of Chemistry
IS - 12
ER -