TY - GEN
T1 - Odor sensing method using olfactory receptors and fluorescent instrumentation
AU - Mujiono, T.
AU - Sukekawa, Y.
AU - Nakamoto, T.
AU - Mitsuno, H.
AU - Kanzaki, R.
AU - Misawa, N.
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/9/8
Y1 - 2015/9/8
N2 - A sensing system based upon a living body's mechanism is useful to improve the performances of current available artificial sensors especially in terms of their selectivity and sensitivity. This paper presents the fundamental study of odor sensor based on insect olfactory receptors (ORs). Cells expressing ORs are responsible for odor detection. It is possible to monitor the increase in calcium ion concentration using the fluorescent protein inside the cells expressing ORs. In our research we developed an instrumentation system to monitor the fluorescent intensity change for the odor sensing. The system consists of fluidic device, sky blue laser for light excitation, lenses, optical filter, dichroic mirror, and a cooled complementary metal oxide semiconductor (CMOS) camera. Sf21 cells expressing two ORs called OR56a and BmOR3 with their specific response to geosmin and bombykal have been prepared for experiments. Measurement results show that the developed instrumentation was able to detect the odorant with concentration down to 5 μM for about 15 seconds.
AB - A sensing system based upon a living body's mechanism is useful to improve the performances of current available artificial sensors especially in terms of their selectivity and sensitivity. This paper presents the fundamental study of odor sensor based on insect olfactory receptors (ORs). Cells expressing ORs are responsible for odor detection. It is possible to monitor the increase in calcium ion concentration using the fluorescent protein inside the cells expressing ORs. In our research we developed an instrumentation system to monitor the fluorescent intensity change for the odor sensing. The system consists of fluidic device, sky blue laser for light excitation, lenses, optical filter, dichroic mirror, and a cooled complementary metal oxide semiconductor (CMOS) camera. Sf21 cells expressing two ORs called OR56a and BmOR3 with their specific response to geosmin and bombykal have been prepared for experiments. Measurement results show that the developed instrumentation was able to detect the odorant with concentration down to 5 μM for about 15 seconds.
KW - Biosensor
KW - Fluorescent instrumentation
KW - Odor sensor
KW - Olfactory receptors
UR - http://www.scopus.com/inward/record.url?scp=84957688275&partnerID=8YFLogxK
U2 - 10.1109/ASCC.2015.7244647
DO - 10.1109/ASCC.2015.7244647
M3 - Conference contribution
AN - SCOPUS:84957688275
T3 - 2015 10th Asian Control Conference: Emerging Control Techniques for a Sustainable World, ASCC 2015
BT - 2015 10th Asian Control Conference
A2 - Selamat, Hazlina
A2 - Ramli, Hafiz Rashidi Haruna
A2 - Faudzi, Ahmad Athif Mohd
A2 - Rahman, Ribhan Zafira Abdul
A2 - Ishak, Asnor Juraiza
A2 - Soh, Azura Che
A2 - Ahmad, Siti Anom
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 10th Asian Control Conference, ASCC 2015
Y2 - 31 May 2015 through 3 June 2015
ER -