Odor sensing method using olfactory receptors and fluorescent instrumentation

T. Mujiono, Y. Sukekawa, T. Nakamoto, H. Mitsuno, R. Kanzaki, N. Misawa

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Citations (Scopus)


A sensing system based upon a living body's mechanism is useful to improve the performances of current available artificial sensors especially in terms of their selectivity and sensitivity. This paper presents the fundamental study of odor sensor based on insect olfactory receptors (ORs). Cells expressing ORs are responsible for odor detection. It is possible to monitor the increase in calcium ion concentration using the fluorescent protein inside the cells expressing ORs. In our research we developed an instrumentation system to monitor the fluorescent intensity change for the odor sensing. The system consists of fluidic device, sky blue laser for light excitation, lenses, optical filter, dichroic mirror, and a cooled complementary metal oxide semiconductor (CMOS) camera. Sf21 cells expressing two ORs called OR56a and BmOR3 with their specific response to geosmin and bombykal have been prepared for experiments. Measurement results show that the developed instrumentation was able to detect the odorant with concentration down to 5 μM for about 15 seconds.

Original languageEnglish
Title of host publication2015 10th Asian Control Conference
Subtitle of host publicationEmerging Control Techniques for a Sustainable World, ASCC 2015
EditorsHazlina Selamat, Hafiz Rashidi Haruna Ramli, Ahmad Athif Mohd Faudzi, Ribhan Zafira Abdul Rahman, Asnor Juraiza Ishak, Azura Che Soh, Siti Anom Ahmad
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479978625
Publication statusPublished - 8 Sept 2015
Externally publishedYes
Event10th Asian Control Conference, ASCC 2015 - Kota Kinabalu, Malaysia
Duration: 31 May 20153 Jun 2015

Publication series

Name2015 10th Asian Control Conference: Emerging Control Techniques for a Sustainable World, ASCC 2015


Conference10th Asian Control Conference, ASCC 2015
CityKota Kinabalu


  • Biosensor
  • Fluorescent instrumentation
  • Odor sensor
  • Olfactory receptors


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