Phytochemical and Antioxidant Activity of Nicotiana tabacum Extracts

Devi A. Putri*, Riyadatus Solihah, Rianur Oktavia, Sri Fatmawati

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Nicotiana tabacum (Solanaceae), also known as tobacco, is one of the most commercialized agricultural crops in the world. However, this plant is not commonly used either as a health treatment or in the discovery of drugs. This study aims to screen phytochemicals and antioxidant activity of N. tabacum leaf extract to find new biomarker compounds for health. The extracts (n-hexane, dichloromethane, ethyl acetate, and acetone extracts) were evaluated for their phytochemical profiles both qualitatively and by the method of total phenol content, while the antioxidant activity of the extracts was determined using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay. Gallic acid was used as the standard. The results showed that the extract contained alkaloids, flavonoids, triterpenoids, phenols, and saponins. The extract had DPPH scavenging activity with a value range of 20.92-30.87 %. Furthermore, dichloromethane extract had the highest DPPH free radical scavenging activity. Interestingly, the highest total phenolic content was also shown in the dichloromethane extract (98.09 g GAE/mg). This study shows that the leaf extract of N. tabacum has the potential as an antioxidant agent.

Original languageEnglish
Title of host publication1st International Seminar on Chemistry and Chemistry Education, ISCCE 2021
EditorsDominggus Malle, Nikmans Hattu, Healthy Kainama, Colincie Natalia Labetubum, I. Wayan Sutapa
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735443044
Publication statusPublished - 11 Jan 2023
Event1st International Seminar on Chemistry and Chemistry Education, ISCCE 2021 - Ambon, Virtual, Indonesia
Duration: 23 Sept 202124 Sept 2021

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


Conference1st International Seminar on Chemistry and Chemistry Education, ISCCE 2021
CityAmbon, Virtual


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