TY - JOUR
T1 - Polymerization of lignosulfonates by the laccase-HBT (1-hydroxybenzotriazole) system improves dispersibility
AU - Nugroho Prasetyo, Endry
AU - Kudanga, Tukayi
AU - Østergaard, Lars
AU - Rencoret, Jorge
AU - Gutiérrez, Ana
AU - del Río, José C.
AU - Ignacio Santos, J.
AU - Nieto, Lidia
AU - Jiménez-Barbero, Jesús
AU - Martínez, Angel T.
AU - Li, Jiebing
AU - Gellerstedt, Göran
AU - Lepifre, Stéphane
AU - Silva, Carla
AU - Kim, Su Yeon
AU - Cavaco-Paulo, Artur
AU - Seljebakken Klausen, Bente
AU - Lutnaes, Bjart Frode
AU - Nyanhongo, Gibson S.
AU - Guebitz, Georg M.
N1 - Funding Information:
Financial support from the BIORENEW EU -project ( NMP2-CT-2006-26456 ), Austrian Academic Exchange Programme (ÖEAD) and the Spanish projects BIO2007-28720-E , BIO2008-01533 , and AGL2008-00709 is acknowledged.
PY - 2010/7
Y1 - 2010/7
N2 - The ability of laccases from Trametes villosa (TvL), Myceliophthora thermophila (MtL), Trametes hirsuta (ThL) and Bacillus subtilis (BsL) to improve the dispersion properties of calcium lignosulfonates 398 in the presence of HBT as a mediator was investigated. Size exclusion chromatography showed an extensive increase in molecular weight of the samples incubated with TvL and ThL by 107% and 572% from 28400 Da after 17 h of incubation, respectively. Interestingly, FTIR spectroscopy, 13C NMR and Py-GC/MS analysis of the treated samples suggested no substantial changes in the aromatic signal of the lignosulfonates, a good indication of the ability of TvL/ThL-HBT systems to limit their effect on functional groups without degrading the lignin backbone. Further, the enzymatic treatments led to a general increase in the dispersion properties, indeed a welcome development for its application in polymer blends.
AB - The ability of laccases from Trametes villosa (TvL), Myceliophthora thermophila (MtL), Trametes hirsuta (ThL) and Bacillus subtilis (BsL) to improve the dispersion properties of calcium lignosulfonates 398 in the presence of HBT as a mediator was investigated. Size exclusion chromatography showed an extensive increase in molecular weight of the samples incubated with TvL and ThL by 107% and 572% from 28400 Da after 17 h of incubation, respectively. Interestingly, FTIR spectroscopy, 13C NMR and Py-GC/MS analysis of the treated samples suggested no substantial changes in the aromatic signal of the lignosulfonates, a good indication of the ability of TvL/ThL-HBT systems to limit their effect on functional groups without degrading the lignin backbone. Further, the enzymatic treatments led to a general increase in the dispersion properties, indeed a welcome development for its application in polymer blends.
KW - Calcium lignosulfonates
KW - Dispersion
KW - Enzymatic modification
KW - Laccase
KW - Polymerization
UR - http://www.scopus.com/inward/record.url?scp=77950342030&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2010.01.048
DO - 10.1016/j.biortech.2010.01.048
M3 - Article
C2 - 20176477
AN - SCOPUS:77950342030
SN - 0960-8524
VL - 101
SP - 5054
EP - 5062
JO - Bioresource Technology
JF - Bioresource Technology
IS - 14
ER -