Preparation and antioxidant activity of methanol extract of myrmecodiarumphii becc

Ant-plant Myrmecodiarumphii Becc. usually used as traditional Papuan medicine. In the present paper, we provide for the first time the cytotoxicity and antioxidant potential of M. rumphiiBecc. This study reported cytotoxic and antioxidant activities of M. rumphiiBecc. by using maceration method for three times in every 24 hours and evaporated to obtain 77 g reddish brown concentrated. All samples were partitioned or fractionated by dichloromethane, ethyl acetate, and methanol fraction, respectively, to obtain 1 to 7 fraction. Cytotoxicity was determined using Brine Shrimp Lethality Test (BSLT) method while the antioxidant activity using 2,2-diphenyl-2-picrylhydrazyl (DPPH) assay. The most active fraction from BSLT test is fraction 7 with LC₅₀ is 0,4752 µg/ml. DPPH assay shows that fraction 5 was the most potentials antioxidant with 92,6601% radical scavenging activity. From this result indicated that the local ant-plant (Sarang Semut plant) from Merauke are high potential as a source of antioxidant.