Pressure effect in supercritical CO2 extraction of plant seeds

Siti Machmudah, Mikako Kondo, Mitsuru Sasaki, Motonobu Goto*, Jun Munemasa, Masahiro Yamagata

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)


Effect of pressure on supercritical carbon dioxide extraction from various seeds was studied. Three kinds of seeds (rosehip, loquat and physic nut) were used as materials. Extraction was conducted at constant temperature (40, 60 and 80 °C), at CO2 flow rate of 3 ml/min and at various pressures (15-9 MPa). Recovery of extract was compared with soxhlet extraction recovery using hexane as solvent. Based on results at constant temperature, the recovery of rosehip seed oil increased with increasing pressure at short extraction time, but decreased as extraction time progressed. Compared with soxhlet extraction, SC-CO2 extraction could extract more rosehip oil higher than soxhlet extraction using hexane. In the extraction of rosehip seed oil, the cross-over region was observed. The recovery of loquat seed oil increased with decreasing pressure at 60 and 80 °C, but at 40 °C, extraction recovery was independent of the pressure. For physic nut, increasing pressure allowed high extraction recovery from 83.7 to 88.7% at constant temperature. However, the SC-CO2 extraction could not completely extract oil from physic nut seeds compared with soxhlet extraction using hexane. The experimental results could be described using two simple models, the partitioning KD model and a two-site kinetic model. The two-site kinetic model applied well at higher pressure compared whereas the KD model failed to correlate the data at high pressure.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalJournal of Supercritical Fluids
Issue number3
Publication statusPublished - Apr 2008
Externally publishedYes


  • Loquat seed
  • Physic nut
  • Pressure effect
  • Rosehip seed
  • Supercritical CO extraction


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