Purification and characterization of thermostable chitinase from Bacillus SW41 for chitin oligomer production

S. Wahyuni; M. T. Suhartono; A. Khaeruni; A. S. Purnomo; Asranudin; Holilah; P. A. Riupassa

doi:10.14233/ajchem.2016.20099

Purification and characterization of thermostable chitinase from Bacillus SW41 for chitin oligomer production

S. Wahyuni^*, M. T. Suhartono, A. Khaeruni, A. S. Purnomo, Asranudin, Holilah, P. A. Riupassa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Bacillus SW41 was isolated from water of shrimp ponds in Maros, South Sulawesi, Indonesia. Molecular identification showed that the isolate belongs to Bacillus group, strains SW41. A chitinase produced by Bacillus SW41 was purified and characterized. The extracellular enzyme was isolated by successive hydrophobic interaction and gel filtration chromatography. The enzyme was shown to have a relative high molecular weight of 130.2 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Through the stages of purification, chitinase could be purified 8.1 fold. Chitinase SW41 showed optimum activity at pH 4 and stable at 60-70°C. As a thermostable enzyme, chitinase SW41 showed t_1/2 relatively long 8.8 h and 8.25 h at 60 and 70°C, respectively. Another unique feature of chitinase SW41 was indicated on the hydrolysis of colloidal chitin and the ability to produce (GlcNAc)-(GlcNAc)5 through HPLC analysis.

Original language	English
Pages (from-to)	2731-2736
Number of pages	6
Journal	Asian Journal of Chemistry
Volume	28
Issue number	12
DOIs	https://doi.org/10.14233/ajchem.2016.20099
Publication status	Published - 2016

Keywords

Bacillus
Chitin
Chitinase
Oligosaccharides

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title = "Purification and characterization of thermostable chitinase from Bacillus SW41 for chitin oligomer production",

abstract = "Bacillus SW41 was isolated from water of shrimp ponds in Maros, South Sulawesi, Indonesia. Molecular identification showed that the isolate belongs to Bacillus group, strains SW41. A chitinase produced by Bacillus SW41 was purified and characterized. The extracellular enzyme was isolated by successive hydrophobic interaction and gel filtration chromatography. The enzyme was shown to have a relative high molecular weight of 130.2 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Through the stages of purification, chitinase could be purified 8.1 fold. Chitinase SW41 showed optimum activity at pH 4 and stable at 60-70°C. As a thermostable enzyme, chitinase SW41 showed t1/2 relatively long 8.8 h and 8.25 h at 60 and 70°C, respectively. Another unique feature of chitinase SW41 was indicated on the hydrolysis of colloidal chitin and the ability to produce (GlcNAc)-(GlcNAc)5 through HPLC analysis.",

keywords = "Bacillus, Chitin, Chitinase, Oligosaccharides",

author = "S. Wahyuni and Suhartono, {M. T.} and A. Khaeruni and Purnomo, {A. S.} and Asranudin and Holilah and Riupassa, {P. A.}",

year = "2016",

doi = "10.14233/ajchem.2016.20099",

language = "English",

volume = "28",

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journal = "Asian Journal of Chemistry",

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T1 - Purification and characterization of thermostable chitinase from Bacillus SW41 for chitin oligomer production

AU - Wahyuni, S.

AU - Suhartono, M. T.

AU - Khaeruni, A.

AU - Purnomo, A. S.

AU - Asranudin,

AU - Holilah,

AU - Riupassa, P. A.

PY - 2016

Y1 - 2016

N2 - Bacillus SW41 was isolated from water of shrimp ponds in Maros, South Sulawesi, Indonesia. Molecular identification showed that the isolate belongs to Bacillus group, strains SW41. A chitinase produced by Bacillus SW41 was purified and characterized. The extracellular enzyme was isolated by successive hydrophobic interaction and gel filtration chromatography. The enzyme was shown to have a relative high molecular weight of 130.2 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Through the stages of purification, chitinase could be purified 8.1 fold. Chitinase SW41 showed optimum activity at pH 4 and stable at 60-70°C. As a thermostable enzyme, chitinase SW41 showed t1/2 relatively long 8.8 h and 8.25 h at 60 and 70°C, respectively. Another unique feature of chitinase SW41 was indicated on the hydrolysis of colloidal chitin and the ability to produce (GlcNAc)-(GlcNAc)5 through HPLC analysis.

AB - Bacillus SW41 was isolated from water of shrimp ponds in Maros, South Sulawesi, Indonesia. Molecular identification showed that the isolate belongs to Bacillus group, strains SW41. A chitinase produced by Bacillus SW41 was purified and characterized. The extracellular enzyme was isolated by successive hydrophobic interaction and gel filtration chromatography. The enzyme was shown to have a relative high molecular weight of 130.2 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Through the stages of purification, chitinase could be purified 8.1 fold. Chitinase SW41 showed optimum activity at pH 4 and stable at 60-70°C. As a thermostable enzyme, chitinase SW41 showed t1/2 relatively long 8.8 h and 8.25 h at 60 and 70°C, respectively. Another unique feature of chitinase SW41 was indicated on the hydrolysis of colloidal chitin and the ability to produce (GlcNAc)-(GlcNAc)5 through HPLC analysis.

KW - Bacillus

KW - Chitin

KW - Chitinase

KW - Oligosaccharides

UR - http://www.scopus.com/inward/record.url?scp=84990888261&partnerID=8YFLogxK

U2 - 10.14233/ajchem.2016.20099

DO - 10.14233/ajchem.2016.20099

M3 - Article

AN - SCOPUS:84990888261

SN - 0970-7077

VL - 28

SP - 2731

EP - 2736

JO - Asian Journal of Chemistry

JF - Asian Journal of Chemistry

IS - 12

ER -

Purification and characterization of thermostable chitinase from Bacillus SW41 for chitin oligomer production

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Keywords

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