Abstract

Recently, Euphorbiaceae have attracted much attention for their potential uses in biodiesel production. Their seeds have been well known as the principal resource for oil production. The seed-derived oil content ranged from 28 % to 39 % by dry weight for all Euphorbiaceae species. Meanwhile, their seed also possesses relatively high protein content, ranged from 22 % to 35 %, making them a new alternative for livestock animal feed resources. Nevertheless, the development of this new animal feed resource is limited to the toxicity of the seeds. Its toxicity is mainly caused by the presence of anti-nutrient agents and toxins, such as curcin, trypsin inhibitor and phorbol ester (PE). The later is known to be tetracyclic diterpenes, which represents the primary toxic substances in one of the Euphorbiaceae plants, Jatropha curcas L. To date, little is known about the biosynthesis of these toxic substances. This research aims to analyze the expression of JcGGPPS gene encoding the geranylgeranyl diphosphate synthase involved in the production of the primary precursor for diterpenoid substances. In addition, the phylogram was presented to show the Euphorbiaceae Casbene Synthase (CS). The RT-PCR analysis showed that JcGGPPS is expressed both in seeds and leaves of J. curcas. Meanwhile, in silico analysis of six Euphorbiaceae species showed that CS from Euphorbia esula, Euphorbia resinifera, Euphorbia peplus and Euphorbia fischeriana are highly identical. In addition, CS from J. curcas derives from a different origin than other Euphorbiaceae plants.

Original languageEnglish
Title of host publicationTowards the Sustainable Use of Biodiversity in a Changing Environment
Subtitle of host publicationFrom Basic to Applied Research - Proceeding of the 4th International Conference on Biological Science, ICBS 2015
EditorsRoy Hendroko Setyobudi, Praptiningsih Gamawati Adinurani, Tri Rini Nuringtyas, Roy Hendroko Setyobudi
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735414013
DOIs
Publication statusPublished - 14 Jun 2016
Event4th International Conference on Biological Science - Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research, ICBS 2015 - Yogyakarta, Indonesia
Duration: 18 Sept 201519 Sept 2015

Publication series

NameAIP Conference Proceedings
Volume1744
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Conference on Biological Science - Towards the Sustainable Use of Biodiversity in a Changing Environment: From Basic to Applied Research, ICBS 2015
Country/TerritoryIndonesia
CityYogyakarta
Period18/09/1519/09/15

Keywords

  • Casbene synthase
  • euphorbiaceae
  • geranylgeranyl diphosphate synthase
  • phorbol ester

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