Secondary metabolites production of epigallocatechin gallate through in vitro culture of camellia sinensis l with cinnamic acid precursors

Sutini*, Widiwurjani, Nora Augustien, Djoko Agus Purwanto, Wirdhatul Muslihatin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

The secondary metabolite epigallocatechin gallate found in the Camellia sinensis L plant, which is bioactive, can be an antioxidant and can prevent cancer. The problem of epigallocatechin gallate presence from plants is dependent on land height, environmental temperature conditions, the need for intensive care, and relatively low production levels. Therefore, we need the technology of epigallocatechin gallate production through in vitro culture techniques. The study aimed to obtain a technique of secondary metabolites production of epigallocatechin gallate through culture in vitro by optimizing the medium and providing precursors. The method used is to initiate callus by adding growth regulators and cinnamic acid precursors. After that, the growth of callus was observed then tested both qualitatively and quantitatively. The result obtained is a compact form of callus containing epigallocatechin gallate. This research suggests that the use of cinnamic acid precursors in vitro culture techniques of Camellia sinensis L in a relatively short period can increase wet callus weight.

Original languageEnglish
Title of host publicationInternational Conference on Life Science and Technology, ICoLiST 2019
EditorsAhmad Taufiq, Hendra Susanto, Hadi Nur, Muhammad Aziz, Chuang-Rung Chang, Hyohyemi Lee, Markus Diantoro, Nandang Mufti, Nik Ahmad Nizam Nik Malek, I. Ching Wang, Djoko Tjahnono Iskandar, Gereon Elbers, Sunaryono Sunaryono, Siti Zubaidah, Sumari Sumari, Aulanni'am, Asep Bayu Nandiyanto, Indra Wibowo, Adeodatus Yuda Handaya
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419902
DOIs
Publication statusPublished - 21 Apr 2020
Event2nd International Conference on Life Science and Technology, ICoLiST 2019 - Malang, East Java, Indonesia
Duration: 12 Sept 201913 Sept 2019

Publication series

NameAIP Conference Proceedings
Volume2231
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference2nd International Conference on Life Science and Technology, ICoLiST 2019
Country/TerritoryIndonesia
CityMalang, East Java
Period12/09/1913/09/19

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