Separation and identification of fatty acid in triacylglycerol isolated from Calophylluminophyllum oil

Nyamplung (Calophylluminophyllum) is one of the mangrove plants. Its seed has a significantly high non-edible oil content of 70.4%. Therefore, most researches were focused on the conversion of this oil into biodiesel. In this work, the proximate composition of C. inophyllum seed, and cis-trans fatty acids of triacylglycerols (TAG) in its oil were investigated. The identification method of double bond position in the corresponding cis and trans isomer was also presented. The position of double bond in fatty acid was determined by characteristic ions. Cis and trans configuration in double bond positions was identified by relative intensity of ions. TAG was obtained in high purity (99%) via batchwise multistage liquid extraction. Fatty acids of TAG identified include palmitic acid (C16:0, 11.37%), palmitoleic acid (C16:1c, 2.65%), margaric acid (C17:0, 0.68%), stearic acid (C18:0, 16.21%), elaidic acid (C18:1t, 0.41%), oleic acid (C18:1c, 32.49%), linoleaidic acid (C18:2t, 0.62%), linoleic acid (C18:2c, 22.99%), linolenic acid (C18:3c, 2.23%), arachidic (C20:0, 6.97%), gondoic acid (C20:1c, 1.74%), and behenic acid (C22:0, 1.64%). It was shown that the proposed method can easily distinguish the double bond position and the cis-trans configuration in oleic, linoleic and linolenic acid methyl esters.