Separation of campesterol and β-sitosterol from a sterol mixture

Yu Hsuan Chuang, Yi Hsu Ju*, Arief Widjaja

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


By solvent crystallization using diethyl ether as the solvent on sterol mixture, brassicasterol and stigmasterol that contains a side chain with double bond were separated from campesterol and β-sitosterol with a saturated side chain. The total campesterol and β-sitosterol content in the liquid phase was more than 97% with a recovery of 12%. Multistage crystallization using acetone as the solvent could increase the recovery of campesterol and β-sitosterol to 30%. By employing zeolite selective adsorption on the campesterol and β-sitosterol fraction, β-sitosterol can be recovered in the liquid phase with a purity of 95.2% and a recovery of 3% (overall recovery 1%). After desorbing in ethanol, campesterol adsorbed on the zeolite can be recovered with a purity of 95.4% and a recovery of 3.7% (overall recovery 1.6%).

Original languageEnglish
Pages (from-to)3027-3038
Number of pages12
JournalSeparation Science and Technology
Issue number13
Publication statusPublished - 1 Sept 2006
Externally publishedYes


  • Adsorption
  • Phytosterol
  • Solvent crystallization
  • Zeolite


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