Soil burial method for plastic degradation performed by Pseudomonas PL-01, Bacillus PL-01, and indigenous bacteria

Maya Shovitri*, Risyatun Nafi'Ah, Titi Rindi Antika, Nur Hidayatul Alami, N. D. Kuswytasari, Enny Zulaikha

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

16 Citations (Scopus)

Abstract

Lately, plastic bag is becoming the most important pollutant for environment since it is difficult to be naturally degraded due to it consists of long hydrocarbon polymer chains. Our previous study indicated that our pure isolate Pseudomonas PL-01 and Bacillus PL-01 could degrade about 10% plastic bag. This present study was aimed to find out whether Pseudomonas PL01 and Bacillus PL01 put a positive effect to indigenous bacteria from marginal area in doing plastic degradation with a soil burial method. Beach sand was used as a representative marginal area, and mangrove sediment was used as a comparison. Plastics were submerged into unsterile beach sand with 10% of Pseudomonas PL-01 or Bacillus PL-01 containing liquid minimal salt medium (MSM) separately, while other plastics were submerged into unsterile mangrove sediments. After 4, 8, 12 and 16 weeks, their biofilm formation on their plastic surfaces and plastic degradation were measured. Results indicated that those 2 isolates put positive influent on biofilm formation and plastic degradation for indigenous beach sand bacteria. Bacillus PL-01 put higher influent than Pseudomonas PL-01. Plastic transparent was preferable degraded than black and white plastic bag 'kresek'. But anyhow, indigenous mangrove soil bacteria showed the best performance in biofilm formation and plastic degradation, even without Pseudomonas PL-01 or Bacillus PL-01 addition. Fourier Transform Infrared (FTIR) analysis complemented the results; there were attenuated peaks with decreasing peaks transmittances. This FTIR peaks indicated chemical functional group changes happened among the plastic compounds after 16 weeks incubation time.

Original languageEnglish
Title of host publicationProceeding of International Biology Conference 2016
Subtitle of host publicationBiodiversity and Biotechnology for Human Welfare
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735415287
DOIs
Publication statusPublished - 26 Jun 2017
Event3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016 - Surabaya, Indonesia
Duration: 15 Oct 2016 → …

Publication series

NameAIP Conference Proceedings
Volume1854
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Biology Conference 2016: Biodiversity and Biotechnology for Human Welfare, IBOC 2016
Country/TerritoryIndonesia
CitySurabaya
Period15/10/16 → …

Keywords

  • Bassilus PL-01
  • Indegenous Microorganism From Beach Sand and Mangrove soil
  • Plastic Degradation
  • Pseudomonas PL-01

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