Study on fluorescence spectra: Characteristics of broiler and pig blood

The fluorescence method has been used for identification of blood disease and blood type of human because of specific characteristics. In this research, we observed the fluorescence characteristics of broiler and pig blood. The blood of broiler and pig were obtained from the local slaughterhouse in Surabaya. Each blood was measured directly using fluorescence spectrophotometer. All experiments were conducted at wavelength of 200 nm to 800 nm with scan speed of 500 nm/min and SlitEx/Em of 10nm/10nm. Fluorescence characteristic of the blood samples was shown from these excitation and emission peaks. From investigation, the broiler bloods have fluorescence spectra at 350.5 nm for excitation and 349.7 and 698.0 nm for emission. Meanwhile, the fluorescence characteristic of pig blood was appeared at 311.0 nm and 309.0; 622.0 nm for excitation and emission, respectively. The results showed that between broiler and pig blood have significant different fluorescence characteristic.