Supercritical anti-solvent micronization of chromatography purified marigold lutein using hexane and ethyl acetate solvent mixture

Panatpong Boonnoun, Hazuki Nerome, Siti Machmudah, Motonobu Goto, Artiwan Shotipruk*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This work aims to study supercritical anti-solvent micronization of marigold derived purified lutein that was dissolved in the mixture of hexane and ethyl acetate (70:30 v/v), the solvent used as the mobile phase for chromatographic purification. The results show significant effect of pressure on the morphology of micronized lutein particles. The increase in lutein initial concentration from 1.5 mg/ml to 3.2 mg/ml and the increase in SC-CO2 flow rate from 15 ml/min to 25 ml/min show no significant effects on the morphology of lutein particles. However, the reduction of mean particle size from about 2 μm to 0.8 μm was observed by increasing SC-CO2 flow rate. The X-ray diffraction patterns of the micronized lutein particles show apparent amorphous nature, while the Fourier transform infrared spectroscopy results show that no chemical structural changes occurred. Moreover, the solubility of the micronized lutein particles in aqueous solution was found to increase significantly from being almost insoluble to having approximately 20% solubility.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalJournal of Supercritical Fluids
Volume80
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Chromatography
  • Lutein
  • Marigold flower
  • Micro-particle
  • Micronization
  • Supercritical anti-solvent

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