TY - JOUR
T1 - Supercritical carbon dioxide extraction of valuable compounds from Citrus junos seed
AU - Ueno, Hiro
AU - Tanaka, Masahiro
AU - Machmudah, Siti
AU - Sasaki, Mitsuru
AU - Goto, Motonobu
N1 - Funding Information:
Acknowledgment This work was partly supported by the Kumamoto University 21st century COE Program “Pulsed Power Science’’.
PY - 2008
Y1 - 2008
N2 - Extraction of Citrus junos seed was carried out at temperatures of 40-70 °C, pressures of 20-50 MPa, and CO 2 flow rate of 3 ml/min with supercritical carbon dioxide to obtain the valuable compounds. Seed oil was also extracted by using Soxhlet extraction with hexane as the solvent during 360 min for comparison with the efficiency of supercritical carbon dioxide extraction. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the components present in the seed oil and Gas chromatography-flame ionization detector (GC-FID) was used to quantify their amounts. Among the conditions studied, the highest extraction yield was obtained at higher pressure and temperature (50 MPa and 70 °C). The extraction yield was about 29.5% of the seed, which was almost comparable to that of hexane Soxhlet extraction (33.8%). The results of the GC-MS analyses showed that the seed oil extracted contained N-methylanthranyl acid methyl, fatty acids (such as palmitic, stearic, oleic, linoleic, and linolenic acid), and physiologically active substances of β-sitosterol and squalene.
AB - Extraction of Citrus junos seed was carried out at temperatures of 40-70 °C, pressures of 20-50 MPa, and CO 2 flow rate of 3 ml/min with supercritical carbon dioxide to obtain the valuable compounds. Seed oil was also extracted by using Soxhlet extraction with hexane as the solvent during 360 min for comparison with the efficiency of supercritical carbon dioxide extraction. Gas chromatography-mass spectrometry (GC-MS) was used to analyze the components present in the seed oil and Gas chromatography-flame ionization detector (GC-FID) was used to quantify their amounts. Among the conditions studied, the highest extraction yield was obtained at higher pressure and temperature (50 MPa and 70 °C). The extraction yield was about 29.5% of the seed, which was almost comparable to that of hexane Soxhlet extraction (33.8%). The results of the GC-MS analyses showed that the seed oil extracted contained N-methylanthranyl acid methyl, fatty acids (such as palmitic, stearic, oleic, linoleic, and linolenic acid), and physiologically active substances of β-sitosterol and squalene.
KW - Fatty acids
KW - N-methylanthranyl acid methyl
KW - Supercritical carbon dioxide extraction
UR - http://www.scopus.com/inward/record.url?scp=77954521888&partnerID=8YFLogxK
U2 - 10.1007/s11947-007-0015-0
DO - 10.1007/s11947-007-0015-0
M3 - Article
AN - SCOPUS:77954521888
SN - 1935-5130
VL - 1
SP - 357
EP - 363
JO - Food and Bioprocess Technology
JF - Food and Bioprocess Technology
IS - 4
ER -