The addition effect of Pseudomonas aeruginosa on biodegradation of methyl orange dye by brown-rot fungus Fomitopsis pinicola

A. S. Purnomo, D. M. Rahayu, R. Nawfa, S. R. Putra

Research output: Contribution to journalConference articlepeer-review

6 Citations (Scopus)

Abstract

The addition effect of Pseudomonas aeruginosa on methyl orange (MO) biodecolorization by Fomitopsis pinicola had been investigated. P. aeruginosa was added into F. pinicola culture at 2, 4, 6, 8, 10 mL (1 mL 5.05 χ 1012 CFU). The addition of 4 ml of P. aeruginosa showed the highest MO biodecolorization approximately 99.53%, while by F. pinicola only was 77.22% in potato dextrose broth (PDB) medium for 7 days incubation. Four metabolites were detected from MO degradation by mixed cultures such as 4-(4-(dimethylimino) hydroxy-cyclohexa-2,5-dien-1-ylidene) hydrazinyl) phenolate; 4-(hydroxy-4-iminio-cyclohexa-2,5-dien-1-ylidene) hydrazinyl) benzene sulfonate; 4-(4-(dimethylimino) methoxy-cyclohexa-2,5-dien-1-ylidene) hydrazinyl) hydroxy-benzene sulfonate; and 4-(hydroxy-4-iminio-cyclohexa-2,5-dien-1-ylidene) hydrazinil) hidroxy-benzene sulfonate. The mixed cultures transformed MO via three pathways: (1) desulfonylation, (2) demethylation, and (3) hydroxylation. These results indicated that P. aeruginosa can enhance MO biodecolorization by F. pinicola.

Original languageEnglish
Article number012008
JournalIOP Conference Series: Materials Science and Engineering
Volume959
Issue number1
DOIs
Publication statusPublished - 30 Oct 2020
Event15th Joint Conference on Chemistry, JCC 2020 - Salatiga, Indonesia
Duration: 9 Sept 2020 → …

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