Abstract

In this study the effect of mixed culture of Zymomonas mobilis and Pichia stipitis to produce bioethanol from Solid Waste Arenga pinnata (SWAP) was investigated. The fermentation liquid substrate was resulted from an integrated process of pretreatment and enzymatic hydrolysis. Combination of diluted acid and ethanol organosolv pretreatment was used to increase the SWAP lignin removal. Raw pretreatment was used to decrease the SWAP particle size to 200 mesh. Acid pretreatment was done using 5% (v/v) diluted sulfate acid. Acid pretreated SWAP was treated for 65 min with organosolv pretreatment. Enzymatic hydrolysis by a combination of cellulase and xylanase was done for 48 h to convert cellulose into reducing sugar. The surfactants (Tween 80) addition was done to increase the sugar yield of the hydrolysis process. Fermentation variable consist of single culture of Z. mobilis as the control and mixed culture of Z. mobilis and P. stipitis, the microorganisms used to convert glucose and xylose into ethanol. The number of inoculum used in this experiment was more than 1.4 billion cells and the duration of fermentation process was 72 h. The delignification process decreased 95.43% lignin in SWAP. X-ray Diffraction (XRD) analysis assay showed an increase of crystallinity index of SWAP with pretreatment combination to 37.87%. Enzymatic hydrolysis by a combination of cellulase and xylanase with the addition of Tween 80 produced 9.16 gr glucose/L reducing sugar concentration. The highest ethanol resulted by fermentation process using mixed culture of Z. mobilis and P. stipitis with 0.33% (v/v) ethanol concentration and 0.57 (g ethanol/g reducing sugar) ethanol yield. Fermentation process using single culture Z. mobilis resulted 0.28% (v/v) ethanol concentration, and 0.48 (g ethanol/g reducing sugar) ethanol yield. The mixed culture fermentation with Z. mobilis and P. stipitis resulted ethanol yield 19 % higher than the single culture fermentation using Z. mobilis.

Original languageEnglish
Title of host publicationSeminar on Materials Science and Technology
EditorsLukman Noerochim
PublisherTrans Tech Publications Ltd
Pages145-150
Number of pages6
ISBN (Print)9783035714340
DOIs
Publication statusPublished - 2019
Event4th International Seminar on Science and Technology, ISST 2018 - Surabaya, Indonesia
Duration: 9 Aug 20189 Aug 2018

Publication series

NameMaterials Science Forum
Volume964 MSF
ISSN (Print)0255-5476
ISSN (Electronic)1662-9752

Conference

Conference4th International Seminar on Science and Technology, ISST 2018
Country/TerritoryIndonesia
CitySurabaya
Period9/08/189/08/18

Keywords

  • Acid pretreatment
  • Bioethanol
  • Enzymatic hydrolysis
  • Fermentation
  • Lignocellulose
  • Organosolv

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