The Identification of Pork Contamination on Beef by Polymerase Chain Reaction (PCR)

Mustofa Helmi Effendi*, Shelma Warda Afdilah, Dhandy Koesoemo Wardhana, Fredy Kurniawan, Rurini Retnowati

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

This study aim was to determine the ability of the Polymerase Chain Reaction (PCR) method for identification of pork contamination. The research sample consisted of beef as a negative control, pork which was diluted and mixed with beef as research material to be examined for pork contamination. The validation test method in this study was by testing the sensitivity in serials of dilution of pork (1:101: 1:103; 1: 105; 1: 107). PCR amplification result showed that this method was able to detect pig DNA contamination in 1: 105 pork dilution. It can be concluded that PCR has the ability to detect pork contamination on beef quite sensitive and accurate.

Original languageEnglish
Pages (from-to)634-637
Number of pages4
JournalSystematic Reviews in Pharmacy
Volume11
Issue number10
DOIs
Publication statusPublished - 2020

Keywords

  • PCR
  • Pig DNA
  • Pork contamination
  • Sensitivity

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