The Influence of Chitosan Concentration on Synthesis of Hydroxyapatite Scaffold on Crystallinity and Surface Morphology

Yudyanto, Hartatiek, R. W. Prasasti, Y. A. Hariyanto, N. Hidayat

Research output: Contribution to journalConference articlepeer-review

6 Citations (Scopus)

Abstract

Hydroxyapatite (HA) is a bioceramic material having a chemical formula (Ca10(PO4)6(OH)2) similar to the chemical structure of bone and hard tissue in humans. Recently, HA-scaffold has been intensively developed by many researchers due to its potential application in dealing with tissue injured. The implantation of HA-scaffold into bone defect aims to help and stimulate the growth of new bone tissue. Crystallinity and microstructure are properties that need to be considered to synthesis HA scaffold. Although, HA is biocompatible and osteoconductive but low biodegradable, to solve these problems added chitosan. Chitosan is a biopolymeric material with specific properties, in terms of biocompatible, non-toxic, osteoconductive, biodegradable, and not carcinogenc. In addition, a natural polymeric chitosan has similarities with the extracellular matrix, in terms of good biological performance and inherent cellular interactions. In this study, HA-scaffold was synthesized using Ca(OH)2 as the raw material of natural calcite mineral, H3PO4, and a solid polyurethane polymer (sponge) as forming of pores by sol-gel method. The addition of chitosan concentration of 0 %, 2 % and 3 % in hydroxyapatite scaffold evaluated its influences on crystallinity and surface morphology. The phase, crystallinity and crystal size are evaluated by XRD. Functional groups in HA scaffold and HA compounds were evaluated with FTIR. The results showed that HA has Ca/P ratio of 1.64. The addition of chitosan concentration caused the decrease of crystallinity and increased the pore size. Meanwhile the crystal sizes were obtained: 49.99 nm (HA), 54.37 nm (HA-scaffold without chitosan), 54.98 nm (HA-scaffold with 2% chitosan), and 40.44 nm (HA-scaffold with 3 % chitosan).

Original languageEnglish
Article number012024
JournalIOP Conference Series: Materials Science and Engineering
Volume367
Issue number1
DOIs
Publication statusPublished - 12 Jun 2018
Externally publishedYes
Event5th International Conference on Advanced Materials Sciences and Technology, ICAMST 2017 - Makassar, Indonesia
Duration: 19 Sept 201720 Sept 2017

Keywords

  • Chitosan
  • HA scaffold
  • and surface morphology
  • crystallinity

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