TY - GEN
T1 - The kinetics of enzymes that involved in cassava fermentation produce by co-culture starter of two lactic acid bacteria
AU - Frediansyah, Andri
AU - Kurniadi, Muhammad
AU - Putri, Nurul Noviandi Nahdia
AU - Prasedya, Eka Sunarwidhi
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/12/23
Y1 - 2019/12/23
N2 - Our previous finding showed that the single culture of Lactobacillus plantarum has a maximum time of 18 hours in order to produce carboxyl cellulose and amylase during cassava fermentation. These two enzymes are important in order to give a final characteristic of modified cassava flour including texture, aroma, flavor and taste. The cyanide content of cassava tuber also decreases during its fermentation process. We therefore aimed to modify the starter using co-culture starter of L. plantarum and L. acidophilusand to analyze the production of extracellular amylase and carboxymethyl cellulose during cassava fermentation. The maximum of carboxymethyl cellulose and amylase activity of 13.76 × 10-3 U/ml and 8.31 × 10-3 U/ml, respectively, were obtained from filtrate that has been incubated at 37°C for 23 and 26 h, respectively under static condition. The Km of CMCase and amylase by co-culture starterof L. plantarum and L. acidophilus (1:1) were 0.4791 × 10-3 U/ml and 0.6133 × 10-3 U/ml, respectively. Vmax for CMCase and amylase were 0.8902 × 10-3 and 0.8703 × 10-3 g/mL, respectively.
AB - Our previous finding showed that the single culture of Lactobacillus plantarum has a maximum time of 18 hours in order to produce carboxyl cellulose and amylase during cassava fermentation. These two enzymes are important in order to give a final characteristic of modified cassava flour including texture, aroma, flavor and taste. The cyanide content of cassava tuber also decreases during its fermentation process. We therefore aimed to modify the starter using co-culture starter of L. plantarum and L. acidophilusand to analyze the production of extracellular amylase and carboxymethyl cellulose during cassava fermentation. The maximum of carboxymethyl cellulose and amylase activity of 13.76 × 10-3 U/ml and 8.31 × 10-3 U/ml, respectively, were obtained from filtrate that has been incubated at 37°C for 23 and 26 h, respectively under static condition. The Km of CMCase and amylase by co-culture starterof L. plantarum and L. acidophilus (1:1) were 0.4791 × 10-3 U/ml and 0.6133 × 10-3 U/ml, respectively. Vmax for CMCase and amylase were 0.8902 × 10-3 and 0.8703 × 10-3 g/mL, respectively.
UR - https://www.scopus.com/pages/publications/85077843035
U2 - 10.1063/1.5141280
DO - 10.1063/1.5141280
M3 - Conference contribution
AN - SCOPUS:85077843035
T3 - AIP Conference Proceedings
BT - Proceedings of the 2nd International Conference on Bioscience, Biotechnology, and Biometrics 2019
A2 - Prasedya, Eka Sunarwidhi
A2 - Martyasari, Ni Wayan Riyani
PB - American Institute of Physics Inc.
T2 - 2nd International Conference on Bioscience, Biotechnology, and Biometrics 2019, ICBBB 2019
Y2 - 13 August 2019 through 14 August 2019
ER -