The utilization of Eschericia coli and Shewanella oneidensis for microbial fuel cell

Microbial Fuel Cell (MFC) is a technology that convert chemical energy into electrical energy with catalytic reaction from microorganism. The research method using bacteria in organic waste on anode compartment and ferricyanide solution on cathode compartment. Wastewater from sugar factory was used as organic waste with bacterial concentration of 10%, 12.5%, 15%, 17.5% (v/v) and with bacteria mixture ratio 1:1, 1:2, 2:1. The result of the research showed that the best voltage of bacteria concentration was 12.5% for Eschericia coli and Shewanella oneidensis bacteria, which were 847 mV and 988 mV, and for the mixed bacteria variable was 1:2 ratio with the voltage was 1261 mV. For 12 days, the largest percentage of the decrease of BOD₅ was 12.5% Eschericia coli bacteria concentration variable reached 84.531% and 17.5% Shewanella oneidensis was 73.779%. The best Fe³⁺ reduction was 53.52% for Escherichia coli at 10% concentration (v/v), and for Shewanella oneidensis bacteria reached out of 62.22% at 15% concentration (v/v). In the variable with mixed bacteria was obtained the best reduction result on the ratio of Eschericia coli : Shewanella oneidensis 1:2 was 77,44%.