The utilization of macroalga and its symbiont bacteria as cellulase enzyme source in the coastal waters of tanjung tiram, south-east Sulawesi, Indonesia

Suhariningsih; Suryani D. Astuti; Herdiani N. Kusumawati; Putri A. Siswanto; Amalia F. Mahmud; Wulan Purnamasari; Fadli Ama

The utilization of macroalga and its symbiont bacteria as cellulase enzyme source in the coastal waters of tanjung tiram, south-east Sulawesi, Indonesia

Suhariningsih, Suryani D. Astuti^*, Herdiani N. Kusumawati, Putri A. Siswanto, Amalia F. Mahmud, Wulan Purnamasari, Fadli Ama

^*Corresponding author for this work

Universitas Airlangga

Research output: Contribution to journal › Article › peer-review

Abstract

The potential use of enzyme produce by macroalga and its symbiont bacteria from Tanjung Tiram has been conducted. This paper explains characteristics of the enzyme produced by microalgae and its symbiont bacteria. The activity revealed that symbiont bacteria were able to produce enzyme cellulose. Methods used were enzyme extraction, enzyme purification by ammonium sulfat precipitation and dialysis, qualitative assay by iodin test, quantitative assay by DNS method and UV-Vis spectrofotometry. Qualitative assay for cellulase activity of macroalgae showed no result, whereas its symbiont bacteria showed cellulase activity. The optimum temperature was 40^oC, pH 7 and salt concentrations were 1 M. Quantitave assay of cellulase activity at optimum condition was 0.077 U/ml. Symbiont bacteria of Macroalgae from Tanjung Tiram were a potential source of cellulase enzyme for industrial purpose.

Original language	English
Pages (from-to)	238-244
Number of pages	7
Journal	Annals of Biology
Volume	36
Issue number	2
Publication status	Published - Apr 2020
Externally published	Yes

Keywords

Cellulase
Macroalgae
Symbiont bacteria
Tanjung Tiram

Cite this

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title = "The utilization of macroalga and its symbiont bacteria as cellulase enzyme source in the coastal waters of tanjung tiram, south-east Sulawesi, Indonesia",

abstract = "The potential use of enzyme produce by macroalga and its symbiont bacteria from Tanjung Tiram has been conducted. This paper explains characteristics of the enzyme produced by microalgae and its symbiont bacteria. The activity revealed that symbiont bacteria were able to produce enzyme cellulose. Methods used were enzyme extraction, enzyme purification by ammonium sulfat precipitation and dialysis, qualitative assay by iodin test, quantitative assay by DNS method and UV-Vis spectrofotometry. Qualitative assay for cellulase activity of macroalgae showed no result, whereas its symbiont bacteria showed cellulase activity. The optimum temperature was 40oC, pH 7 and salt concentrations were 1 M. Quantitave assay of cellulase activity at optimum condition was 0.077 U/ml. Symbiont bacteria of Macroalgae from Tanjung Tiram were a potential source of cellulase enzyme for industrial purpose.",

keywords = "Cellulase, Macroalgae, Symbiont bacteria, Tanjung Tiram",

author = "Suhariningsih and Astuti, {Suryani D.} and Kusumawati, {Herdiani N.} and Siswanto, {Putri A.} and Mahmud, {Amalia F.} and Wulan Purnamasari and Fadli Ama",

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T1 - The utilization of macroalga and its symbiont bacteria as cellulase enzyme source in the coastal waters of tanjung tiram, south-east Sulawesi, Indonesia

AU - Suhariningsih,

AU - Astuti, Suryani D.

AU - Kusumawati, Herdiani N.

AU - Siswanto, Putri A.

AU - Mahmud, Amalia F.

AU - Purnamasari, Wulan

AU - Ama, Fadli

PY - 2020/4

Y1 - 2020/4

N2 - The potential use of enzyme produce by macroalga and its symbiont bacteria from Tanjung Tiram has been conducted. This paper explains characteristics of the enzyme produced by microalgae and its symbiont bacteria. The activity revealed that symbiont bacteria were able to produce enzyme cellulose. Methods used were enzyme extraction, enzyme purification by ammonium sulfat precipitation and dialysis, qualitative assay by iodin test, quantitative assay by DNS method and UV-Vis spectrofotometry. Qualitative assay for cellulase activity of macroalgae showed no result, whereas its symbiont bacteria showed cellulase activity. The optimum temperature was 40oC, pH 7 and salt concentrations were 1 M. Quantitave assay of cellulase activity at optimum condition was 0.077 U/ml. Symbiont bacteria of Macroalgae from Tanjung Tiram were a potential source of cellulase enzyme for industrial purpose.

AB - The potential use of enzyme produce by macroalga and its symbiont bacteria from Tanjung Tiram has been conducted. This paper explains characteristics of the enzyme produced by microalgae and its symbiont bacteria. The activity revealed that symbiont bacteria were able to produce enzyme cellulose. Methods used were enzyme extraction, enzyme purification by ammonium sulfat precipitation and dialysis, qualitative assay by iodin test, quantitative assay by DNS method and UV-Vis spectrofotometry. Qualitative assay for cellulase activity of macroalgae showed no result, whereas its symbiont bacteria showed cellulase activity. The optimum temperature was 40oC, pH 7 and salt concentrations were 1 M. Quantitave assay of cellulase activity at optimum condition was 0.077 U/ml. Symbiont bacteria of Macroalgae from Tanjung Tiram were a potential source of cellulase enzyme for industrial purpose.

KW - Cellulase

KW - Macroalgae

KW - Symbiont bacteria

KW - Tanjung Tiram

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M3 - Article

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SN - 0970-0153

VL - 36

SP - 238

EP - 244

JO - Annals of Biology

JF - Annals of Biology

IS - 2

ER -

The utilization of macroalga and its symbiont bacteria as cellulase enzyme source in the coastal waters of tanjung tiram, south-east Sulawesi, Indonesia

Abstract

Keywords

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