Two-dimensional digital lock-in circuit for fluorescent imaging of odor biosensor system

Yuji Sukekawa, Totok Mujiono, Takamichi Nakamoto

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

In the field of life science, fluorescent imaging techniques are often adopted to investigate activities of cells. Previously, we have proposed the application of fluorescent imaging for the odor sensing system. The system relays a temporal change in odorant concentration via a change in fluorescent intensity by utilizing a cell with an olfactory receptor and a fluorescent protein. However, the system needs to be robust against ambient light as the fluorescent intensity is relatively weak. Additionally, the system should process the data in real time. In this study, many digital lock-in amplifiers were implemented into a field programmable gate array. An image taken from the image sensor was divided into 100 small areas where lock-in amplifiers parallelly worked in real time. The effect of the lock-in measurement for the image was demonstrated. Measurements of the cells under the different ambient light conditions confirmed that the system removed the influence of ambient light intensity. The developed system is capable of supporting the application of fluorescent imaging in real time sensing systems such as odor sensors.

Original languageEnglish
Title of host publicationProceedings - 2017 1st New Generation of CAS, NGCAS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages241-244
Number of pages4
ISBN (Electronic)9781509064472
DOIs
Publication statusPublished - 26 Sept 2017
Externally publishedYes
Event1st New Generation of CAS, NGCAS 2017 - Genova, Italy
Duration: 6 Sept 20179 Sept 2017

Publication series

NameProceedings - 2017 1st New Generation of CAS, NGCAS 2017

Conference

Conference1st New Generation of CAS, NGCAS 2017
Country/TerritoryItaly
CityGenova
Period6/09/179/09/17

Keywords

  • FPGA
  • Fluorescent imaging
  • Lock-in detection
  • Odor biosensor

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