Valorization of Solid Food Waste as a Source of Polyunsaturated Fatty Acids Using Aurantiochytrium sp. L3W

Toshikazu Suenaga, Satoshi Nakai*, Akira Umehara, Wataru Nishijima, Takehiko Gotoh, Nurlaili Humaidah

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Purpose: This study aimed at valorizing solid food waste containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Methods: Aurantiochytrium sp. L3W that produces DHA and EPA was cultivated on eight types of solid food waste: sake lees (SL), crown daisy, Japanese mustard spinach (JMS), soy sauce residue, lemon peel (LP), orange peel, grape skin, and Hiroshimana old pickle (HOP). The biomass mixture of the remaining food waste and strain L3W was analyzed for DHA and EPA. To characterize the types of food waste, the leachability of dissolved organic carbon (DOC) and dissolved nitrogen (DN) was compared. Results: The strain L3W grew on both pasteurized and unsterilized food waste such as SL and JMS. Elution of DOC and DN from the food waste might be a factor affecting the growth of strain L3W. However, the strain L3W might utilize solid-state organic compounds in JMS. Despite the unsterile conditions, the biomass mixture of SL contained both DHA and EPA, whereas DHA was found in the biomass mixtures of JMS, LP and HOP, thereby confirming the valorization of these types of solid food waste. Unsterile mass cultivation of the strain L3W using SL and HOP in a 200 L tank also produced a biomass mixture containing 12.6 mg-DHA/g and 0.217 mg-EPA/g. These DHA and EPA contents were 1500-times and 37-times higher, respectively, than that in commercial poultry feed, indicating that the biomass mixtures could be used as an additive in poultry feed. Graphical Abstract: [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)2945-2956
Number of pages12
JournalWaste and Biomass Valorization
Issue number9
Publication statusPublished - Sept 2023


  • Alternative fish oil
  • Aurantiochytrium sp
  • Polyunsaturated fatty acids
  • Solid food waste
  • Unsterile cultivation


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