Zingiber officinale Var. rubrum extract increases the cytotoxic activity of 5-fluorouracil in colon adenocarcinoma widr cells

Sarmoko*, Iin Solihati, Joko Setyono, Heny Ekowati, Arif Fadlan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

One of the main chemotherapy agents used to treat colon cancer is 5- fluorouracil (5-FU). However, over the years, the effectiveness of this medication has significantly decreased, therefore, co-chemotherapy with another agent is needed to enhance the activity. This study evaluates the effect of Zingiber officinale var Rubrum (red ginger) extract as a cochemotherapy agent with 5-FU in the WiDr human colorectal cancer cell line. The MTT assay used to determine the cytotoxic effect for 24 and 48 hours. A 48 hours treatment of 5-FU in WiDr cells showed an IC50 value of 130 μg/mL, with none obtained during the 24 hours treatment. Evaluation of the extract for 24 and 48 hours exhibited a cytotoxic effect of 68 μg/mL and 65 μg/mL, respectively. Furthermore, the addition of extract with a concentration less than half of their IC50 caused a significant decrease in cell viability than a single application 5-FU after 48 hours incubation. In conclusion, red ginger extract increases the cytotoxic activity of 5-FU in colon adenocarcinoma WiDr cells.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalIndonesian Journal of Pharmacy
Volume31
Issue number4
DOIs
Publication statusPublished - 29 Dec 2020

Keywords

  • 5-Fluorouracil
  • Chemotherapy
  • Cytotoxic effect
  • WiDr cell lines
  • Zingiber officinale var Rubrum

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